Design the forward and reverse primers to mutate

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Design the forward and reverse primers to mutate

Design the forward and reverse primers to mutate the followingcodons (just the internal primers, not the outside primers). Mutation primers are usually 20 nucleotides long, only design thento 9 nucleotides. Underline the mutant codon, and include at least3 nucleotides from each side of the primer so that the final primeris 9 nucleotides long.
5′ -TAAGGCTAGTAGACCGTATGCCGAATAC-3′
Mutate the threonine to alanine.

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