Two hybrid system to detect interactions between proteins

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Two hybrid system to detect interactions between proteins

Using the two-hybrid system to detect interactions between proteins, you obtain the following results: A clone encoding gene A gave positive results with clones B and C; clone Bgave positive results with clones A, D, and E but not C; and clone E gave positive results only with clone B. Another clone, F, gave positive results with clone G but not with any of A-E.

Can you explain these results?

a. Protein A binds to proteins D, E, F and G; protein B binds to proteins C, F, and G; protein C binds to protein B, C, D, E, F and G; protein Dand E both bind to protein A, C, F and G; protein G and F bind to A, B, C, D and E.

b. Protein A binds to proteins D, E and C; protein B binds to proteins A, D, and E; protein C binds only to protein A; protein D and E both bind only to protein A; protein G and F bind only to each other.

c. Protein A binds to proteins B and C; protein B binds to proteins A, D, and E; protein C binds only to protein A; protein D and E both bind only to protein B; protein G and F bind only to each other.

d. None of all above

 

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