What are limitations to resolution in light microscope

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What are limitations to resolution in light microscope

1. What are the major limitations to resolution in a light microscope? and how does usingoil increase resolution?

2. Which do you think is more accurate detector of theequivalence point: a color indicator or a pH electrode andmeter?Why

3. Describe how the myelin sheath is formed in the PNS. How are the nodes of Ranvier formed? What is the relationshipbetween the myelin sheath and the neurilemma?

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