Q. What differences in detail do you notice in freshly prepared versus permanently mounted tissue? How did the steps of preparations (with fresh microtome samples or staining and fixing of permanent mounts) affect the appearance of the samples? Tabulate some advantages and disadvantages of each. What component of root-tip cells is stained by Safranin in the permanent mount of a longitudinal section of a corn root tip? What component of root-tip cells does Fast Green stain? What could different stains such as these indicate with respect to cell division, differentiation, and function?