What the gel would look like with all three digest

Home Blog What the gel would look like with all three digest

What the gel would look like with all three digest

You have a circular plasmid containing 9kb of DNA, and you wish to map its EcoR1 and BamHI sites. When you digest the plasmid with EcoRI and run the resulting DNA on a gel, you observe a single band at 9kb. You get the same result when you digest the DNA with BamHI. When you digest with a mixture of both enzymes, you observe two bands, one 6kb and the other 3kb in size. Explain these results. Draw both a picture of what the gel would look like with all three digest and a map of the restriction sites.

Add comment

Academic Research Pro